puri ed mouse ig isotype standard page 9 39  (SouthernBiotech)

Journal: Scientific Reports

Article Title: Outer membrane vesicles from flagellin-deficient Salmonella enterica serovar Typhimurium induce cross-reactive immunity and provide cross-protection against heterologous Salmonella challenge

doi: 10.1038/srep34776

Figure Lengend Snippet: The total amount of anti-LPS ( a ) or -OMP ( b ) IgG in the sera obtained from mice immunized intranasally with OMVs, the total amount of anti-LPS ( c ) or -OMP ( d ) IgG in the sera obtained from mice immunized intraperitoneally with OMVs, and the total amount of S-IgA that was specific for LPS ( e ) or for OMPs ( f ) were measured using quantitative ELISA. Each group consisted of 10 (control) or 12 (vaccinated) mice. The mice were immunized with OMVs that were derived from S. Typhimurium and then boosted at week 5. Samples were collected at 4 weeks and 8 weeks after the first immunization. PBS-vaccinated mice served as the control group. The data shown represent the concentration of IgG or S-IgA antibodies in samples obtained from mice and are shown according to standard curves.

Article Snippet: To obtain standard curves for each antibody isotype, plates were coated in triplicate with two-fold dilutions of the appropriate purified mouse Ig isotype standard IgG, IgG 1 , IgG 2a and IgA (BD Biosciences, San Jose, CA, USA), starting at 0.5 μg/μl.

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Concentration Assay

Journal: Scientific Reports

Article Title: Outer membrane vesicles from flagellin-deficient Salmonella enterica serovar Typhimurium induce cross-reactive immunity and provide cross-protection against heterologous Salmonella challenge

doi: 10.1038/srep34776

Figure Lengend Snippet: Serum IgG 1 and IgG 2a responses in intranasally ( a ) and intraperitoneally ( b ) immunized and control mice. Outer membrane proteins (OMPs) as the coated immunogen. The data represented ELISA results determining the concentration of IgG 1 and IgG 2a subclass antibody to OMPs in the serum of mice immunized by intranasal or intraperitoneal route with flagellin-deficient OMVs. Each group has 10 or 12 mice. Mice were boosted at week 5 and blood samples were collected on 4 weeks and 8 weeks after first immunization. PBS-vaccinated mice were in the control group. The error bars represented variations between triplicate wells.

Article Snippet: To obtain standard curves for each antibody isotype, plates were coated in triplicate with two-fold dilutions of the appropriate purified mouse Ig isotype standard IgG, IgG 1 , IgG 2a and IgA (BD Biosciences, San Jose, CA, USA), starting at 0.5 μg/μl.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay